Add to ORKGof infected slices, we counted the number of neurons containing 1-Gal, and estimated that 68 to 95 % of neurons in the injected region were infected, de-pending on whether central or peripheral regions of virus injection were scrutinized. Stained slices were photographed (Zeiss Axiophot, x2.5 and x10 ob-jectives) and imported into Adobe Photoshop 3.0 for graphic presentation. 19. Hippocampal slices (500 pLm) were prepared from young adult male Sprague-Dawley rats. Slices were submerged in a stream of artificial cerebral spinal fluid (ACSF) (119 mMNaCI, 2.5 mMKCI, 1.3 mM MgSO4 2.5mM CaCI2, 1.0mM Na2HPO4,26.2mM NaH3C0, and 11.0 mM glucose), maintained at 222C to5°C, and gassed with 95%02/5 % 002. fEPSPs measured independently in SR or SO at
<p>Acute hippocampal slices (400 μm thick) were prepared from 4- to 8-week-old Wister rats and a sli...
<p>The number of infected neurons in 24 coronal planes sampling the neuraxis 5 days following inject...
<p>Photomicrographs of toluidine blue-stained hippocampal neurons in CA3 (A-D), CA4 (G–J), and the u...
Hippocampal slice preparation. Hippocampal slices were obtained from wistar rats (12-17 days old). A...
<p><b>A</b>–<b>D</b>: Fluorescence photomicrographs demonstrating immunoreactivity against N protein...
<p>DIV 5 GFP-neurons were injected unilaterally into the right hippocampus of 6 weeks old rats using...
Virus-mediated gene transfer into neurons is a powerful tool for the analysis of neuronal structure ...
Hippocampal slices have long been used to investigate prop-erties of synaptic transmission and plast...
<p>TPLSM images: <b>A–D</b>) brain slicing performed 2 h after intravenous injection of SR101 (20 mg...
face:!5.7 mm,!5.5 mm,!5.3 mm). A micromanipulator (Narishige) was used to control injection speed at...
AbstractHere we describe a technique that uses a recombinant vaccinia virus to transfect neurons in ...
<p>Recombinant RCMV allowing expression of GFP (green) in the infected cells, was injected intravent...
<p>Series of coronal sections (organized from rostral to caudal) for a rat surviving three days afte...
To detect subtle changes in neuronal morphology in response to changes in experience, one must image...
<p>(A) Schematic of the experimental protocol. Rats were given intra-LA infusion of either AAV-shSCR...
<p>Acute hippocampal slices (400 μm thick) were prepared from 4- to 8-week-old Wister rats and a sli...
<p>The number of infected neurons in 24 coronal planes sampling the neuraxis 5 days following inject...
<p>Photomicrographs of toluidine blue-stained hippocampal neurons in CA3 (A-D), CA4 (G–J), and the u...
Hippocampal slice preparation. Hippocampal slices were obtained from wistar rats (12-17 days old). A...
<p><b>A</b>–<b>D</b>: Fluorescence photomicrographs demonstrating immunoreactivity against N protein...
<p>DIV 5 GFP-neurons were injected unilaterally into the right hippocampus of 6 weeks old rats using...
Virus-mediated gene transfer into neurons is a powerful tool for the analysis of neuronal structure ...
Hippocampal slices have long been used to investigate prop-erties of synaptic transmission and plast...
<p>TPLSM images: <b>A–D</b>) brain slicing performed 2 h after intravenous injection of SR101 (20 mg...
face:!5.7 mm,!5.5 mm,!5.3 mm). A micromanipulator (Narishige) was used to control injection speed at...
AbstractHere we describe a technique that uses a recombinant vaccinia virus to transfect neurons in ...
<p>Recombinant RCMV allowing expression of GFP (green) in the infected cells, was injected intravent...
<p>Series of coronal sections (organized from rostral to caudal) for a rat surviving three days afte...
To detect subtle changes in neuronal morphology in response to changes in experience, one must image...
<p>(A) Schematic of the experimental protocol. Rats were given intra-LA infusion of either AAV-shSCR...
<p>Acute hippocampal slices (400 μm thick) were prepared from 4- to 8-week-old Wister rats and a sli...
<p>The number of infected neurons in 24 coronal planes sampling the neuraxis 5 days following inject...
<p>Photomicrographs of toluidine blue-stained hippocampal neurons in CA3 (A-D), CA4 (G–J), and the u...