Germination of Colletotrichum lagenarium By IWAO FURUSAWA, MASAMICHI NISHIGUCHI,

The biochemical events of spore germination of Colletotrichum lagenarium were investi-gated. Cycloheximide (3-55,UM) inhibited spore germination when added during the first 40 min of incubation, but was ineffective when added after 40 min. Spores germinated when cycloheximide was removed from the suspension for 2 h during incubation, regardless of the length of pretreatment with cycloheximide. By pulse-labelling with [1*C]glycine, it was evident that the protein which was synthesized during the first 40 min of incubation was indispensable for the initiation of germination, but protein synthesis which occurred at a later stage of incubation was not necessary for spore germination directly