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Objective: Groin pain commonly affects football players and can be associated with prolonged recovery periods. Understanding the relationship between groin pain and reliable measures of hip flexibility and strength may facilitate the development of optimal rehabilitation and prevention strategies. In this study, the reliability and association with athletic groin pain of hip flexibility and strength measures were investigated. Methods: A cohort of 29 football players (15–21 years) participating in junior elite competitions (Australian Rules football and soccer) were recruited. The intra-rater reliability (n = 13) and inter-rater reliability (n = 12) of various hip flexibility (bent knee fall out test, hip internal rotation, hip external rotation) and strength (hip abduction, hip interna