Simultaneous Determination of Seven Synthetic Colorants in Wine by Dispersive Micro-Solid-Phase Extraction Coupled with Reversed-Phase High-Performance Liquid Chromatography

A novel and effective dispersive micro-solid-phase extraction (d-m-SPE) using ethanediamine-functionalized magnetic Fe3O4 polymer (EDA-MP) as an efficient adsorbent in wine sample was developed. Based on this, a simple and time-saving analytical method for the simultaneous determination of seven synthetic colorants (i.e., tartra-zine, amaranth, carmine, sunset yellow, allura red, brilliant blue and erythrosine) in wine by reversed-phase high-performance liquid chro-matography with an ultraviolet detector was established. The exper-imental parameters, including the chromatographic retention behavior of studied synthetic colorants, the effect of the usage amount of cross-linking monomer, the effect of the usage amount of EDA-MP on the recovery and the recyclability of the adsorbents, were studied in detail. The results showed that the EDA-MP could be reused efficiently at least six times. Under optimized conditions, the recoveries for all analytes were in the range of 88.6–105.2%, with the intraday relative standard deviations (RSDs) ranging from 2.1 to 8.2 % and the interday RSDs ranging from 3.4 to 8.7%, and all the analytes had good linearities in the tested ranges with correlation coefficients (r2)>0.9995. The limits of quantification for seven syn-thetic colorants were between 0.12 and 0.45 mg L21. The developed method was successfully applied to wine samples, and it was con-firmed that the EDA-MP particles were highly effective d-m-SPE materials