Accurate quantification of more than 4,000 mouse tissue proteins reveals minimal proteome changes during aging. Mol. Cell Proteomics

The biological process of aging is believed to be the result of an accumulation of cellular damage to biomol-ecules. Although there are numerous studies address-ing mutation frequencies, morphological or transcrip-tional changes in aging mammalian tissues, few have measured global changes at the protein level. Here, we present an in depth proteomic analysis of three brain regions as well as heart and kidney in mice aged 5 or 26 months, using stable isotope labeling of whole animals (SILAC mouse) and high resolution mass spectrometry. In the frontal cortex and hippocampal regions of the brain, more than 4200 proteins were quantitatively com-pared between age groups. Proteome differences be-tween individual mice were observable within and be-tween age groups. However, mean protein abundanc