It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast. However, we show that {Delta}mto1 cells, which are defective in nucleation of cytoplasmic microtubules, have profoundly misoriented spindles but are not delayed in the timing of sister chromatid separation, providing compelling evidence that fission yeast does not possess a spindle orientation checkpoint. Instead, we show that latrunculin A delays anaphase onset by disrupting interpolar microtubule stability. This effect is abolished in a latrunculin A-insensitive actin mutant and exacerbated in cells lacking Ase1, which cross-links antiparallel interpolar microtubules at ...
AbstractBackground: Proteins conserved from yeast to human hold two sister chromatids together. The ...
To ensure genome stability during mitosis, the mitotic spindle must segregate sister chromosomes acc...
SummaryMany microtubule (MT) structures contain dynamic MTs that are bundled and stabilized in overl...
International audienceIn animal and yeast cells, the mitotic spindle is aligned perpendicularly to t...
It has previously been proposed that in fission yeast mitotic spindle position is monitored by a che...
Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting...
International audienceIn all eukaryotes, the alignment of the mitotic spindle with the axis of cell ...
This paper is made available online in accordance with publisher policies. Please scroll down to vie...
AbstractThe spindle orientation checkpoint (SOC) in fission yeast has been proposed to delay metapha...
In the budding yeast Saccharomyces cerevisiae, the mitotic spindle must align along the mother-bud a...
Segregating genetic material along the longest axis of the cell ensures that there is a sufficient d...
Accurate chromosome segregation relies on the bipolar mitotic spindle. In many eukaryotes, spindle f...
International audienceFaithful segregation of sister chromatids requires the attachment of each kine...
Microtubules are involved in a wide range of cellular functions including the establishment of cell ...
The onset of anaphase is triggered by activation of the anaphase promoting complex/cyclosome (APC/C)...
AbstractBackground: Proteins conserved from yeast to human hold two sister chromatids together. The ...
To ensure genome stability during mitosis, the mitotic spindle must segregate sister chromosomes acc...
SummaryMany microtubule (MT) structures contain dynamic MTs that are bundled and stabilized in overl...
International audienceIn animal and yeast cells, the mitotic spindle is aligned perpendicularly to t...
It has previously been proposed that in fission yeast mitotic spindle position is monitored by a che...
Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting...
International audienceIn all eukaryotes, the alignment of the mitotic spindle with the axis of cell ...
This paper is made available online in accordance with publisher policies. Please scroll down to vie...
AbstractThe spindle orientation checkpoint (SOC) in fission yeast has been proposed to delay metapha...
In the budding yeast Saccharomyces cerevisiae, the mitotic spindle must align along the mother-bud a...
Segregating genetic material along the longest axis of the cell ensures that there is a sufficient d...
Accurate chromosome segregation relies on the bipolar mitotic spindle. In many eukaryotes, spindle f...
International audienceFaithful segregation of sister chromatids requires the attachment of each kine...
Microtubules are involved in a wide range of cellular functions including the establishment of cell ...
The onset of anaphase is triggered by activation of the anaphase promoting complex/cyclosome (APC/C)...
AbstractBackground: Proteins conserved from yeast to human hold two sister chromatids together. The ...
To ensure genome stability during mitosis, the mitotic spindle must segregate sister chromosomes acc...
SummaryMany microtubule (MT) structures contain dynamic MTs that are bundled and stabilized in overl...