Protocol Imaging Cell Movements in Egg-Cylinder Stage Mouse Embryos

Cell movements in the pregastrulation egg cylinder mouse embryo play an important role in pattern-ing. The stereotypic movement of the anterior visceral endoderm converts a proximal-distal axis to an anteroposterior axis by properly positioning the primitive streak. The epiblast at this stage is also characterized by a great deal of cell mixing, about which very little is known. Visualizing such cel