Speciation of Arsenic ions (As+3/ As+5) with the yeastimmobilized column

The speciation study of trace metals in analytical science is getting more attention in these days because different toxicity and chemical properties are shown depending on their oxidation states and elements combined with.1 Arsenic has been widely used in industrial and agricultural products for a long period. After being absorbed in bio-organisms, it could be converted in various species such as monomethyl and dimethyl As. Inorganic arsenic species is more toxic than organic species and As3+ shows at least 10 times higher toxicity than As5+.2 Consequently, investigations have been performed on arsenic oxidation states as well as organic species.3-10 Gas Chromatography (GC)11,12 and High-performance liquid chromatography (HPLC)10,13-15 have been success-fully used for the separation of arsenic species. Anionic1