Genome Sequence of Staphylococcus capitis QN1, Which Causes Infective Endocarditis

Staphylococcus capitis is a subtype of coagulase-negative staphylococci (CoNS) which could emerge as a significant pathogen causing infective endocarditis, prosthetic valve endocarditis, and late-onset sepsis. We isolated S. capitis strain QN1 from the skin swab sample of a female. Here we prepared a genome sequence for this strain consisting of 30 contigs totaling 2,430,101 bases and a GC content of 32.76%. Staphylococcus capitis is a subtype of coagulase-negative staph-ylococci (CoNS), and it is part of the normal bacterial flora of humans and has been occasionally reported in infective endocar-ditis and prosthetic valve endocarditis cases (5, 10). Recent reports indicate its emergence as a significant pathogen causing late-onset sepsis in very-low-birth-weight (VLBW) infants (1,500 g) (8). The pathogenesis of CoNS is mainly due to their ability to form biofilms on indwellingmedical devices, which confers tolerance to disinfectants during surgery (12). However, there is very little in-formation about the pathogenesis of S. capitis. We isolated S. ca-pitis QN1 from the skin swab sample of a female in The First Affiliated Hospital of Zhejiang University at Hangzhou, China. The isolate was identified with the MicroFlex LT instrument (Bruker Daltonics) according to the manufacturer’s protocols. Spectra were analyzed by using Flexcontrol 3.0 software and the Biotyper 2.0 database (Bruker Daltonics) (9). The taxonomy of this isolate also has been confirmed by 16S rRNAgene sequencing. The sample was prepared for sequencing by growing S. capitis QN1 aerobically overnight at 37°C inMueller-Hinton broth (Ox-oid). Genomic DNA was extracted using the DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s recom-mended protocol. The quantity of DNA obtained was determined using a Nanodrop spectrophotometer and a Qubit fluorometer. Typically, 5 g of DNA was then sent to the State Key Laborator