THE EFFECT OF CONCENTRATION OF CARRIER, pH AND TIME OF EXTRACTION ON SEPARATION'S FACTOR OF PENICILLIN G- PHENYL ACETATE BY REACTIVE EXTRACTION

The aim of this research is to study the effect of concentration carrier, pH and time of extraction on separation's factor of penicillin g- phenyl acetate by reactive extraction technique. The 10 mL aqueous solution with variation of pH: 5, 6 contains 0.001 M penicillin G and 0.001 M phenyl acetate has been extracted with 10 mL n-butyl acetate contains dioctylamine as carrier. Variation concentration of carrier were 0.000; 0.002; 0.004; 0.006 and 0.008 M. Variation time of extraction were 1, 5, 10, 15 and 20 min. The penicillin G and phenyl acetate that dissolved in organic phase ha been reextracted with 10 mL aqueous with variation of pH: 7, 8. The optimum condition obtained as follow: concentration dioctylamine was 0.002M; pH the first phase water was 5 and the second phase water was 8; and the time of extraction was 10 min