Title: Determination of Selected Secondary and Tertiary Amine Alkaloids in Barley Malt /7 Abstract approved:

nomethylindole (gramine) are known precursors for N-nitrosamines. Analytical procedures were developed and applied to extract and quantify these alkaloids from malt, green malt, malt roots, and raw barley. A ground malt sample under nitrogen atmosphere was extracted with 5 percent concentrated ammonium hydroxide in meth-anol at room temperature for 24 hours. After purification, the extracted alkaloids were quantitated by reverse phase high per-formance liquid chromatography using both phosphate buffer and ion-pairing solvent systems. The recoveries obtained for the al-kaloids listed were, respectively, 77.4, 92.0, 74.0 and 80.5 per-cent with detection limit of 0.2, 0.2, 0.1 and 0.1 pg/gm. Con-formation of identity of each alkaloid was accomplished by TLC, UV, and MS. Twenty-three samples of kilned malts, five samples of green malts, ten samples of kilned malt roots, five samples of gree