REVIEW Studies of Human Airways in vitro: A Review of the Methodology

The pathophysiology of human airway na~owing is only partly understood. In order to gain more insight in the mechanisms of human lung diseases and potential beneficial therapeutic agents, adequate models are needed. Animal airway models are of limited value since lung diseases such as asthma and chronic obstructive pulmonary disease (COPD) are unique to humans and because the mechanisms of airway narrowing differ between species. There-fore, it is important to perform studies on human isolated airways. We describe the models that have been developed to study airway function in vitro, emphasizing human airway preparations. The easily prepared airway strip and ring preparations are described first. The potential damage during preparation and the interference with airway structure are important drawbacks in these preparations. Lung parenchymal strips, described next, were designed in order to study responsiveness of small airways. However, parenchymal strips are anatomically complex, and responsiveness is determined by the relative amounts of airway and vascular smooth muscle. The lack of reproducibility between species and even within one animal limits their usefulness. Airway tube preparations, in which luminal and serosal stimulation can be separated, enable us to study the modulatory rote of the airway