Tumor-Associated Antigen in Human Pancreatic Cancer 1.2

saline extracts of 1) a human pancreatic cancer cell line (MIA PaCa-2) 2) human pancreatic tumors excised from Swiss nulnu mice, or 3) human pancreatic cancer tissue. Solid-phase Immu-noadsorbents rendered the resulting antisera specific. The anti-sera, tested by double immunodiffusion and counterimmunoelec-trophoresis (CEP), detected the pancreatic antigen in saline ex-tracts of human pancreatic carcinoma, human fetal pancreas, MIA PaCa-2 cells, a second human pancreatic cancer cell line (PANC-1), and nude mouse pancreatic tumors, but not in saline extracts of normal human pancreas and a number of other normal tissues, In normal human sera, or In sera from patients with active inflammatory disease. The antisera did not react with alpha-fetoprotein and carclnoembryonlc antigen. Sera of patients with pancreatic cancer and other neoplastic and nonneoplastic disorders were tested in CEP with two antisera: rabbit anti-MI