Separation of YbdN bioactive protein from Bacillus subtilis isolated from the Red Sea algae Sargassum sp. with bioactivity against antibiotic resistant bacterial pathogens

Abstract. Hundreds of bacterial isolates from Red Sea Jeddah coast included seawater, sediments, algae (Halimeda sp., Sargassum sp), sponges (Siphonochalina sp., Leucetta chgosensis), sea cucumber, (Holothuria atra), star fish (Acanthaster planci), mangrove (Avecenia marina) roots, sea anemone, (Heteractis magnifica), and jelly fish. One bacterial culture of them isolated from algae Sargassum sp identified as B. subtilis produced YbdN protein which known as antimicrobial compound against Methicillin-Resistant Staphylococcus aureus, Vancomycin-Resistant Enterococci and Listeria monocytogenes. B. subtilis production of YbdN protein was best in Colombia broth with pieces of synthetic polyurethane sponge at 28°C incubation temperature for 7 days, and shaking at 210 rpm