qnrB19 Gene Bracketed by IS26 on a 40-Kilobase IncR Plasmid from

an Escherichia coli Isolate from a Veal Calf qnrB19 genes have been reported in Escherichia coli, Esch-erichia hermannii, Salmonella enterica, and Klebsiella spp., lo-cated on IncN, IncL/M (human isolates), and ColE-like (both human and chicken isolates) plasmids (2, 6, 8, 9, 11, 13, 14, 16). This study describes the characterization of the genetic envi-ronment of a plasmid-mediated qnrB19 gene identified in E. coli isolated from a veal calf in the Netherlands. E. coli strain 013.1 was selected from a fecal sample grown on a MacConkey agar plate supplemented with 0.125 mg/liter ciprofloxacin. This strain showed MIC values of 0.25 mg/liter and 16 mg/liter for ciprofloxacin and nalidixic acid, respec-tively, suggesting the presence of a plasmid-mediated quino-lone resistance mechanism (10, 18). PCR as described for qnrA and qnrS (4), qnrB (3), qnrC (20)