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blastocysts derived from control and GSH-treated spermatozoa were diploid, whereas blastocysts derived fromDTT-treated spermatozoa genome of the founder offsprings. Moreover, ICSI makes male pronucleus formation have been suspected asit possible to introduce large DNA molecules such asDOI: 10.1530/REP-08-0143yeast or mammalian artificial chromosomes (O250 q 2009 Society for Reproduction and Fertilitythe two pivotal factors for causing the low develop-mental capacity of ICSI-fertilized porcine embryoswere haploid. In conclusion, sperm treatment with GSH enhanced the developmental capacity of porcine embryos produced by our optimized ICSI procedure. Reproduction (2009) 137 633–64