Simultaneous simulation of two different concentration time curves in vitro

A model for in-vitro simultaneous simulation of two different patterns of pharmacokinetic parameters was developed. In the first compartment the initial concentration simulates that seen after intravenous infusion or bolus injection and the antibiotic is diluted at a constant rate. In the second compartment the concentration rises from zero to a maximum of { the maximum of the first concentration. From this second compartment the drug is diluted at a slower rate. In previous studies the bactericidal activity of antibiotics was evaluated under conditions simulating serum pharmacokinetic parameters (Shah, 1980). In this model the initial concentration was as high as the peak serum concentration after an intravenous injection, exposing the bacteria to very high antibiotic concentrations. The model used was further developed to study simultaneously two patterns of continuously changing antibiotic concentrations, firstly that seen in serum and an intravenous dose, and secondly where concentrations begin at zero, gradually rise to a peak of i serum-peak and then gradually decline. Metho