A Fourier transform infrared spectroscopic comparison of cultured human fibroblast and fibrosarcoma

Several advantages of infrared vibration spectroscopy made it a promising technique in both the study and diagnosis of cancer. These advantages include: infrared light has a low absorption and scattering coefficient, which might be able to allow deeper penetration of infrared radiation into the tissue sample as it is very crucial for in-vivo diagnosis. Also, vibration spectra provide fingerprints of chemical groups present in cells which can help us in getting the information on the molecular level. In the present work, Fourier-transform infrared spectroscopy was used to compare cultured human fibroblast and malignant fibrosarcoma cells. Significant differences were observed by comparing the spectra of the normal cells with that of the cancer cells. The P02 symmetric stretching mode at 1082cm is shifted to a higher frequency in cancer cells and a broad band, whose center is located at 1064 cm ' in the cancer cell is reduced in intensity. These observations are similar to those reported previously by Wong et al in comparing the JR spectra of pairs of normal and cancerous cells from the colon and cervix. However, the observed increase in the relative intensity of the symmetric to antisymmetric CH3 bending mode are only found i