A Recombinant DNA Plasmid which Inhibits Bacteriophage T7 Reproduction in Escherichia coli

C. Stone
R Ichard
D. Smith
R. C. Miller

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Publication date

January 1983

DOI

10.1099/0022-1317-64-7-1615

ISSN

0022-1317

Citation count (estimate)

Abstract

Escherichia coli plasmids carrying a DNA restriction fragment corresponding to the extreme right end of the T7 bacteriophage nome render cells incompetent to support reproduction of wild-type T7. Processing of intracellular concatemeric phage DNA and cell lysis are perturbed by one such p|asmid, pRS148. The results are discussed with reference to the possibility that the right end of the T7 genome ncodes a lysis-related function