Time-Dependent Degradation Pattern of

used for the diagnosis of acute myocardial infarction (AMI). However, it is still unclear whether degraded cTnT forms circulate in the patient’s blood. We there-fore aimed to elucidate which cTnT forms are detected by the clinical assay. METHODS: Separation of cTnT forms by gel filtration chromatography (GFC)was performed in sera from 13 AMI patients to examine cTnT degradation. The GFC eluates were subjected toWestern blot analysis with the original antibodies from the Roche immunoassay used to mimic the clinical cTnT assay. To investigate the degradation pattern with time, standardized serum samples of 18 AMI patients collected 0–72 h after ad-mission were analyzed by Western blot analysis. RESULTS: GFC analysis of AMI patients ’ sera revealed