Development of a Love-wave Immunosensor with Microfluidic Technology to Detect Phages in Dynamic Mode

The goal has been to develop an immunosensor based on a Love-wave device and a PDMS microfluidic chip in order to detect potentially pathogenic microorganisms in real time. Therefore, it has been tested with the phage M13 detected through the mouse monoclonal antibody anti-M13 (AM13). The Double-layer Agar (DLA) technique has been used to quantify the number of phages, linked to the sensor surface, in plaques-forming unit (pfu); that has allowed us to compare the number of phages determined by DLA and the estimated by the frequency shift, and both were very similar. With the aim to obtain a short response time, the detection of the phage was carried out in dynamic mode. The range tested concentrations has varied between 2x1010 pfu·ml-1 and 5x108 pfu·ml-1. A fast response and a good discrimination among concentrations have been achieved. Besides, the AM13 has been used as secondary antibody in order to measure lower concentrations, such as 5·107 pfu·ml-1