COMPARATIVE STUDY OF PLASMA MEMBRANE INTEGRITY OF SPERMATOZOA BY USING HOS MEDIUM AND DISTILLED WATER AND THEIR RELATION WITH POST THAW MOTILITY

This study was carried out on 148 frozen thawed semen samples from cattle (58 from 11 HF bulls, 36 from 7 crossbred bulls, 33 from 6 Sahiwal bulls and 21 from 4 Jersey bulls) and 57 frozen thawed semen samples from 11 buffalo bulls maintained at Animal Breeding Centre (a Unit of ABRO India), Salon, Rae Bareli, UP. Plasma membrane integrity of cryopreserved sperms was studied with the help of HOS solution (Osmolarity 0.080 Osmole/ kg, pH 7.31) and double distilled water (Osmolarity 0.001 Osmole/kg, pH 5.93). The mean percentages of HOS reactive sperms in HOS solution and double distilled water for cattle and buffalo were 57.61±0.57, 48.98±0.34 and 57.06±0.75, 47.14±0.34, respectively. Except, Crossbred, the percentage of HOS reactive sperms in HOS solution was significantly (P<0.05) higher than the percentage of reactive sperms in distilled water in HF, Jersey and Sahiwal breed of cattle. The results revealed that the HOS solution was more suitable hypo-osmotic medium for assessing the plasma membrane integrity of frozen thawed spermatozoa as compared to Double distilled water. Highly significant positive correlations were observed between HOS reactive sperms in HOS solution and in distilled water as well as with post thaw motility (PTM) in both the species (r = 0.42 to 0.75)