ORIGINAL ARTICLE Double Transgenic Pigs with Combined Expression of Human a1,2-Fucosyltransferase and a-Galactosidase Designed to Avoid

Abstract Hyperacute rejection (HAR) depends on the response of xenoreactive antibodies principally against porcine a-Gal epitope. Methods eliminating HAR include GGTA1 inactivation, regulation of the complement system and modification of the oligosaccharide structure of surface proteins in donor’s cells. Transgenic animals designed for the purpose of xenotransplantation with single modification do not display full reduction of the a-Gal epitope level, which means that a accumulation of several modifications in one transgenic individual is needed. The aim of the study was to create a molecular and cytogenetic profile of a double transgenic animal with a1,2-fucosyltransferase and a-galactosidase expression. As a result of interbreeding of an individual with a1,2-fucosyltransferase expression with an individual with a-galactosidase expression 12 livin