RESEARCH ARTICLE Topical Formulation Containing Naringenin: Efficacy against Ultraviolet B Irradiation- Induced Skin Inflammation and Oxidative Stress in Mice

Naringenin (NGN) exhibits anti-inflammatory and antioxidant activities, but it remains unde-termined its topical actions against ultraviolet B (UVB)-induced inflammation and oxidative stress in vivo. The purpose of this study was to evaluate the physicochemical and functional antioxidant stability of NGN containing formulations, and the effects of selected NGN con-taining formulation on UVB irradiation-induced skin inflammation and oxidative damage in hairless mice. NGN presented ferric reducing power, ability to scavenge 2,20-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) and hydroxyl radical, and inhibited iron-inde-pendent and dependent lipid peroxidation. Among the three formulations containing NGN, only the F3 kept its physicochemical and functional stability over 180 days. Topical applica-tion of F3 in mice protected from UVB-induced skin damage by inhibiting edema and cyto-kine production (TNF-α, IL-1β, IL-6, and IL-10). Furthermore, F3 inhibited superoxide anion and lipid hydroperoxides production and maintained ferric reducing and ABTS scavengin