Methods

Currently, the use of stem cells from permanent (DPSCs) and deciduous teeth (SHEDs) has enabled many therapeutic advances. Despite numerous sources of stem cells, DPSCs and SHEDs have shown several advantages, since the isolation method is noninvasive and cells present rapid expansion in vitro [1]. DPSCs have shown the capacity to form a dentin-pulp like complex. Due to its high osteogenic potential, these cells have been applied around dental implants in order to form new bone tissue [2-4]. Given the clinical rele-vance in developing research towards DPSCs, this study aimed to isolate stem cells from dental pulp tissue using the explant technique