Candidate Biomarker Discovery for Angiogenesis b Spectral- and X!

Abstract Candidate protein biomarker discovery by full automatic integration of Orbitrap full from previous Fourier transform ion cyclotron resonance (FT-ICR) peptide profiling. Lower mass pensated by the larger number of detected masses separated by liquid chromatography (LC), which can be directly linked to protein identifications. The number of peptide sequences divided by the ulated due to tailing in LC retention time (40%), modifications (40%) and mass determination tant role in proteomics research. Orbitrap combines great mass mixtures separated by nano-liquid chromatography (nano-slightly better mass accuracy of 6 1 ppm [1]. However, the per-formance of FT-ICR depends on the magnet strength chosen, e.g., 9.4 T, and thus it takes up a lot more space because of the sizeable magnet. Orbitrap has a shorter time scale of measur-ing than FT-ICR and can therefore be more conveniently linked to a nano-LC column to measure peptide fractions of a trypsin-digested sample. Comparison of mass spectra from different samples is called peptide profiling. Peptide profiling creates a matrix of * Corresponding author