ORAL PRESENTATION Open Access ISWI and CHD chromatin remodelers bind to promoters but act in gene bodies

ATP-dependent nucleosome remodelers influence genetic processes by altering nucleosome occupancy, positioning, and composition. In vitro, yeast imitation switch (ISWI) and chromodomain helicase DNA-binding (CHD) remodelers bind ~30-85 bp of extranucleosomal DNA [1-3]. However, in vivo, ISWI and CHD remodelers act within gene bodies [4], which contain regularly spaced nucleosomes separated by less than 20 bp of lin-ker DNA [5]. How, then, do ISWI and CHD remodelers act within regions containing insufficient linker DNA for their association with chromatin? Methods To resolve this apparent paradox, we have mapped the genomic distributions of the yeast Isw1, Isw2, and Chd1 remodelers at base-pair resolution using immunoprecipita