Cardiac High-Energy Phosphate Metabolism Alters with Age as Studied in 196 Healthy Males with the Help of 31- Phosphorus 2-Dimensional Chemical Shift Imaging

Recently published studies have elucidated alterations of mitochondrial oxidative metabolism during ageing. The intention of the present study was to evaluate the impact of ageing on cardiac high-energy phosphate metabolism and cardiac function in healthy humans. 31-phosphorus 2-dimensional chemical shift imaging (31P 2D CSI) and echocardiography were performed in 196 healthy male volunteers divided into groups of 20 to 40 years (I, n = 43), 40 to 60 years (II, n = 123) and. 60 years (III, n = 27) of age. Left ventricular PCr/b-ATP ratio, myocardial mass (MM), ejection fraction and E/A ratio were assessed. Mean PCr/b-ATP ratios were significantly different among the three groups of volunteers (I, 2.1060.37; II, 1.7760.37; III, 1.4560.28; all p,0.001). PCr/b-ATP ratios were inversely related to age (r2 =20.25; p,0.001) with a decrease from 2.65 by 0.02 per year of ageing. PCr/b-ATP ratios further correlated with MM (r =20.371; p,0.001) and E/A ratios (r = 0.213; p,0.02). Moreover, E/A ratios (r =20.502, p,0.001), MM (r = 0.304, p,0.001), glucose-levels (r = 0.157, p,0.05) and systolic blood pressure (r = 0.224, p,0.005) showed significant correlations with age. The ejection fraction did not significantly differ between the groups. This study shows that cardiac PCr/b-ATP ratios decrease moderately with age indicating an impairment of mitochondrial oxidative metabolism due to age. Furthermore, MM increases, and E/A ratio decreases with age. Both correlate with left-ventricular PCr/b-ATP ratios. The findings of the present study confir