Construction of synthetic genetic networks requires the assembly of DNA fragments encoding functional biological parts in a definedorder. Yet thismaybecomea time-consumingprocedure. To address this technical bottleneck,wehave created a series of Gateway shuttle vectors and an integration vector, which facilitate the assembly of artificial genes and their expression in the buddingyeastSaccharomyces cerevisiae.Ourmethodenables the rapidconstructionof anartificial gene fromapromoter andan open reading frame (ORF) cassette by one-step recombination reaction in vitro. Furthermore, the plasmid thus created can readilybe introduced intoyeast cells to test theassembledgene’s functionality.As flexible regulatory componentsof a synthetic genetic netw...
<p>Yeast expression vectors can be created by one-step recombination reaction using a promoter entry...
Bacterial operons are nature’s tool for regulating and coordinating multi-gene expression in prokary...
We have constructed new yeast vectors for targeted integration and conditional expression of any seq...
Construction of synthetic genetic networks requires the assembly of DNA fragments encoding functiona...
Assembly of synthetic genetic circuits is central to synthetic biology. Yeast <i>S. cerevisiae</i>, ...
Background: In vivo recombination of overlapping DNA fragments for assembly of large DNA constructs ...
We have adapted the Golden Gate DNA assembly method to the assembly of transcription units (TUs) for...
We have adapted the Golden Gate DNA assembly method to the assembly of transcription units (TUs) for...
Synthetic biology is founded on the idea that cells are living machines that execute genetically enc...
The tractability of the budding yeast genome has provided many insights into the fundamental mechani...
Conventional methods for covalent immobilization of proteins often result in denaturation due to che...
Most conditional expression vectors designed for mammalian cells have been valuable systems for stud...
The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of r...
Background: Recombinatorial cloning using the Gateway (TM) technology has been the method of choice ...
A set of shuttle vectors was constructed to facilitate expression of genes for metabolic engineering...
<p>Yeast expression vectors can be created by one-step recombination reaction using a promoter entry...
Bacterial operons are nature’s tool for regulating and coordinating multi-gene expression in prokary...
We have constructed new yeast vectors for targeted integration and conditional expression of any seq...
Construction of synthetic genetic networks requires the assembly of DNA fragments encoding functiona...
Assembly of synthetic genetic circuits is central to synthetic biology. Yeast <i>S. cerevisiae</i>, ...
Background: In vivo recombination of overlapping DNA fragments for assembly of large DNA constructs ...
We have adapted the Golden Gate DNA assembly method to the assembly of transcription units (TUs) for...
We have adapted the Golden Gate DNA assembly method to the assembly of transcription units (TUs) for...
Synthetic biology is founded on the idea that cells are living machines that execute genetically enc...
The tractability of the budding yeast genome has provided many insights into the fundamental mechani...
Conventional methods for covalent immobilization of proteins often result in denaturation due to che...
Most conditional expression vectors designed for mammalian cells have been valuable systems for stud...
The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of r...
Background: Recombinatorial cloning using the Gateway (TM) technology has been the method of choice ...
A set of shuttle vectors was constructed to facilitate expression of genes for metabolic engineering...
<p>Yeast expression vectors can be created by one-step recombination reaction using a promoter entry...
Bacterial operons are nature’s tool for regulating and coordinating multi-gene expression in prokary...
We have constructed new yeast vectors for targeted integration and conditional expression of any seq...