Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluorescent proteins can easily be inserted into yeast genes at their chromosomal locus, by homologous recombination, for expression of tagged proteins at endogenous levels. This is especially useful for incorporation of multiple fluorescent protein fusions into a single strain, which can be challenging in organisms where genetic manipulation is more complex. How-ever, the availability of optimal fluorescent protein combinations for 3-color imaging is lim-ited. Here, we have characterized a combination of fluorescent proteins, mTFP1/mCitrine/ mCherry for multicolor live cell imaging in S. cerevisiae. This combination can be used with conventional blue...
AbstractTagging expressed proteins with the green fluorescent protein (GFP) from Aequorea victoria[1...
Microscopy has served as a crucial tool for the advancement of biological knowledge, such as discove...
doi:10.3791/3454 (2012). Several microscopy techniques are available today that can detect a specifi...
Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluoresce...
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of prote...
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of prote...
Summary: Tracking the inheritance patterns of proteins (TrIPP) is a live-cell imaging technique used...
Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet t...
<p>Recommended fluorescent protein combinations for imaging in yeast are broken down by filter set (...
Fluorescent proteins (FPs) are widely used in many organisms, but are commonly characterised in vitr...
Transcription factor-based biosensors represent promising tools in the construction and evaluation o...
Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet t...
For the investigation of protein localization and trafficking using live cell imaging, researchers o...
The authors report on a method for the multicolor imaging of cell surface proteins which is based on...
<div><p>To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critica...
AbstractTagging expressed proteins with the green fluorescent protein (GFP) from Aequorea victoria[1...
Microscopy has served as a crucial tool for the advancement of biological knowledge, such as discove...
doi:10.3791/3454 (2012). Several microscopy techniques are available today that can detect a specifi...
Saccharomyces cerevisiae are widely used for imaging fluorescently tagged protein fusions. Fluoresce...
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of prote...
Fluorescent protein fusions are a powerful tool to monitor the localization and trafficking of prote...
Summary: Tracking the inheritance patterns of proteins (TrIPP) is a live-cell imaging technique used...
Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet t...
<p>Recommended fluorescent protein combinations for imaging in yeast are broken down by filter set (...
Fluorescent proteins (FPs) are widely used in many organisms, but are commonly characterised in vitr...
Transcription factor-based biosensors represent promising tools in the construction and evaluation o...
Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet t...
For the investigation of protein localization and trafficking using live cell imaging, researchers o...
The authors report on a method for the multicolor imaging of cell surface proteins which is based on...
<div><p>To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critica...
AbstractTagging expressed proteins with the green fluorescent protein (GFP) from Aequorea victoria[1...
Microscopy has served as a crucial tool for the advancement of biological knowledge, such as discove...
doi:10.3791/3454 (2012). Several microscopy techniques are available today that can detect a specifi...