Research Article Macrophage Derived Platelet Activating Factor Implicated in the Resolution Phase of Gouty Inflammation

which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Human blood derived in vitro differentiated monocytes or macrophages are a population of cells which have been investigated over the years to determine the role these cells play in the resolution phase of gout. Macrophages are able to phagocytose monosodium urate monohydrate (MSU) crystals without releasing inflammatory factors. This study analysed macrophage platelet activating factor secretion and its possible role in the pathway of gout resolution.Analysis of sunatants from in vitrodifferentiatedmacrophages stimulated withMSU crystals revealed the secretion of platelet activating factor (PAF) 1.54±0.10mean ± SEM; ng/mL per 106 cells. This secretion was absent in immature monocytes treated similarly. When these monocytes were pretreated with recombinant human PAF-acetylhydrolase (rhuPAF-AH) and MSU crystals resulted in TN