Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagating material are essential for the management of pospiviroid diseases in horticultural crops. This study describes the development of a true multiplexed diagnostic method for the detection and identification of all nine currently recognized pospiviroid species in one assay using Luminex bead-based suspension array technology. In addition, a new data-driven, statistical method is presented for establishing thresholds for positivity for individual assays within multiplexed arrays. When applied to the multiplexed array data generated in this study, the new method was shown to have better control of false positives and false negative results than...
For molecular diagnostics, assays detecting biomarkers can be used to provide information to medical...
For molecular diagnostics, assays detecting biomarkers can be used to provide information to medical...
Pospiviroids are plant pathogens, composed only of infectious RNA molecules. They are both latent on...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
<p>Performance of the 11-plex bead-based array when screened against a large panel of single and mix...
Bead-based suspension array systems enable simultaneous fluorescence-based identification of multipl...
Bead-based suspension array systems enable simultaneous fluorescence-based identification of multipl...
Bead-based suspension array systems enable simultaneous fluorescence-based identification of multipl...
For molecular diagnostics, assays detecting biomarkers can be used to provide information to medical...
<p>One sample of <i>Potato spindle tuber viroid</i> (isolate #3077695) was tested in ten independent...
For molecular diagnostics, assays detecting biomarkers can be used to provide information to medical...
For molecular diagnostics, assays detecting biomarkers can be used to provide information to medical...
Pospiviroids are plant pathogens, composed only of infectious RNA molecules. They are both latent on...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagat...
<p>Performance of the 11-plex bead-based array when screened against a large panel of single and mix...
Bead-based suspension array systems enable simultaneous fluorescence-based identification of multipl...
Bead-based suspension array systems enable simultaneous fluorescence-based identification of multipl...
Bead-based suspension array systems enable simultaneous fluorescence-based identification of multipl...
For molecular diagnostics, assays detecting biomarkers can be used to provide information to medical...
<p>One sample of <i>Potato spindle tuber viroid</i> (isolate #3077695) was tested in ten independent...
For molecular diagnostics, assays detecting biomarkers can be used to provide information to medical...
For molecular diagnostics, assays detecting biomarkers can be used to provide information to medical...
Pospiviroids are plant pathogens, composed only of infectious RNA molecules. They are both latent on...