The suitability of laboratory-bred

Background: A stenogamous colony of Anopheles cracens (A. dirus B) established 20 years ago in a Thai insectary proved susceptible to Plasmodium vivax. However, routine sporozoite production by feeding on field‑collected blood samples has not been described. The setting‑up of an A. cracens colony in an insectary on the Thai‑Myanmar border and the process of using P. vivax field samples for the production of infectious sporozoites are described. Methods: The colony was started in 2012 from egg batches that were sent from the Department of Parasitology, Fac‑ ulty of Medicine, University of Chiang Mai, to the Shoklo Malaria Research Unit (SMRU), on wet filter paper in sealed Petri dishes. From May 2013 to December 2014, P. vivax‑infected blood samples collected from patients seeking care at SMRU clinics were used for membrane feeding assays and sporozoite production. Results: Mosquitoes were fed on blood samples from 55 patients, and for 38 (69 %) this led to the production sporozoites. The average number of sporozoites obtained per mosquito was 26,112 (range 328–79,310). Gametocy‑ taemia was not correlated with mosquito infectiousness (p = 0.82), or with the number of the sporozoites produced (Spearman’s ρ = −0.016, p = 0.905). Infectiousness did not vary with the date of collection or the age of the patient. Mosquito survival was not correlated with sporozoite load (Spearman’s ρ = 0.179, p = 0.282). Conclusion: Consistent and routine P. vivax sporozoites production confirms that A. cracens is highly susceptible to P. vivax infection. Laboratory‑bred colonies of this vector are suitable for experimental transmission protocols and thus constitute a valuable resource