Genotyping of carbapenem resistant Acinetobacter baumannii isolated from tracheal tube discharge of hospitalized patients in intensive care

Background and Objectives: Carbapenem resistant A. baumannii is an emerging cause of nosocomial infections. The aims of this study were identification of the most prevalent of carbapenem resistant genes, molecular typing and antimicrobial evaluation of A.baumannii in intensive care units. Materials and Methods: Two hundred and six A. baumannii were isolated from tracheal tube discharge of hospitalized patients at different intensive care units in Ahvaz, Iran. Antimicrobial susceptibility test was done on all isolates. Multiplex and singleplex PCR were performed for detection of blaOXA-23-like, blaOXA-24-like, blaOXA-51-like,blaOXA-58-like, blaVIM, blaIMP, blaSPM and bla NDM genes. Genetic relationship of all isolates was determined by REP-PCR method. Results: Out of 206 examined isolates, 198 (96.1%) isolates were resistant to imipenem and meropenem. However 3.9% isolates were sensitive to these antibiotics. The blaOXA-23-like and blaOXA-24-like genes were detected in 85 % and 8.7 % of strains, respectively. No blaOXA-58- like, blaIMP, blaVIM, blaSPM and blaNDM were detected. REP-PCR results showed that isolates were belonged to five genotypes: Genotype A was the most prevalent (P- value < 0.001): it was observed in 75 of 206 strains (36.4%). Genotype B, and C were found in 28.6 % and 27.7%, respectively. The rate of other genotypes was as follows: