POSTER PRESENTATION Open Access The 4-D landscape of the inflammatory response

It is widely accepted that chromatin ‘responds ’ to physiolo-gical cues via protein:DNA interactions and nucleosome rearrangement [1,2], and that transcription plays a key role in its higher-order organization [3]. What remains elusive is how the nuclear landscape reshapes, in 3-D space and time, to facilitate such responses to unfold. Materials and methods We add tumour necrosis factor a (TNFa) to primary human endothelial cells and induce the inflammatory cascade; this is orchestrated by the transcription factor NF-B [4]. We monitor the response for 0-85 min post-induction using ChIP nucleosome-positioning studies, and chromosome conformation capture, all coupled to next-generation sequencing. We also apply a new approach, where the isolation of ‘transcription factories ’ [5] i