Research Article Degradation Potential of Protocatechuate 3,4-Dioxygenase from Crude Extract of Stenotrophomonas maltophilia Strain KB2 Immobilized in Calcium Alginate

Copyright © 2014 Urszula Guzik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Microbial intradiol dioxygenases have been shown to have a great potential for bioremediation; however, their structure is sensitive to various environmental and chemical agents. Immobilization techniques allow for the improvement of enzyme properties.This is the first report onuse of glyoxyl agarose and calciumalginate asmatrixes for the immobilization of protocatechuate 3,4-dioxygenase. Multipoint attachment of the enzyme to the carrier caused maintenance of its initial activity during the 21 days. Immobilization of dioxygenase in calciumalginate or on glyoxyl agarose resulted in decrease in the optimum temperature by 5∘Cand 10∘C, respectively. Entrapment of the enzyme in alginate gel shifted its optimum pH towards high-alkaline pHwhile immobilization of the enzyme on glyoxyl agarose did not influence pHprofile of the enzyme. Protocatechuate 3,4-dioygenase immobilized in calciumalginate showed increased activity towards 2,5-dihydroxybenzoate, caffeic acid, 2,3-dihydroxybenzoate, and 3,5-dihydroxybenzoate. Slightly lower activity of the enzyme was observed after its immobilization on glyoxyl agarose. Entrapment of the enzyme in alginate gel protected it against chelators and aliphatic alcohols while its immobilization on glyoxyl agarose enhanced enzyme resistance to inactivation by metal ions. 1