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Introduction: The objective of this study was to study the epidemiological characteristics and the microbiological profile of patients suspected with microbial keratitis in Gujarat. Methods: Corneal scraping was collected from 200 consecutive cases of suspected microbial keratitis and was subjected to direct examination and culture. Results: Of the 200 ulcers 55 % were culture positive, 26.5 % were bacterial ulcers of which 47% were due to Staphylococcus spp. Pure fungal growth was seen in 22 % while 6 % were mixed ulcers. Fusarium spp. (30%) was the most common fungus followed by Aspergillus spp. (21%). Only one case of Acanthamoeba keratitis was encountered. Patients were mainly from rural area