Plasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesicular transport. However, PfRab5B is unique in lacking a C-terminal geranyl-geranylation motif, while having N-terminal palmitoylation and myristoylation motifs. We show that the N-terminal glycine is required for PfRab5B myristoylation in vitro and when an N-terminal PfRab5B fragment possessing both acylation motifs is fused to GFP and expressed in transgenic P. falciparum parasites, the chimeric PfRab5B protein localizes to the plasma membrane. Upon substitution of the modified glycine by alanine the staining becomes diffuse and GFP is found in soluble subcellular fractions. Immuno-electron microscopy shows endogenous PfRab5B decorating the parasite’s plasma and...
Rab genes encode a subgroup of small GTP-binding proteins within the ras super-family that regulate ...
Spatial and temporal distribution of the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase...
International audienceOver-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schiz...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
<div><p><i>Plasmodium falciparum</i> (Pf) has a family of 11 Rab GTPases to regulate its vesicular t...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
As a first step towards developing a set of compartment-specific probes for studying protein traffic...
As a first step towards developing a set of compartment-specific probes for studying protein traffic...
Background information. The pathology causing stages of the human malaria parasite Plasmodium falcip...
Background information. The pathology causing stages of the human malaria parasite Plasmodium falcip...
Over-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schizonts produces a puncta...
Rab genes encode a subgroup of small GTP-binding proteins within the ras super-family that regulate ...
Rab genes encode a subgroup of small GTP-binding proteins within the ras super-family that regulate ...
Spatial and temporal distribution of the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase...
International audienceOver-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schiz...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
<div><p><i>Plasmodium falciparum</i> (Pf) has a family of 11 Rab GTPases to regulate its vesicular t...
International audiencePlasmodium falciparum (Pf) has a family of 11 Rab GTPases to regulate its vesi...
As a first step towards developing a set of compartment-specific probes for studying protein traffic...
As a first step towards developing a set of compartment-specific probes for studying protein traffic...
Background information. The pathology causing stages of the human malaria parasite Plasmodium falcip...
Background information. The pathology causing stages of the human malaria parasite Plasmodium falcip...
Over-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schizonts produces a puncta...
Rab genes encode a subgroup of small GTP-binding proteins within the ras super-family that regulate ...
Rab genes encode a subgroup of small GTP-binding proteins within the ras super-family that regulate ...
Spatial and temporal distribution of the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase...
International audienceOver-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schiz...