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Objective: Calcium hydroxide is the most widely used intracanal medicament in en-dodontics, which should be removed prior to permanent root canal filling to minimize its intervention with the bonding of endodontic sealers. This ex-vivo study aimed to evaluate the effect of pretreatment with calcium hydroxide on the bond strength of Resilon/Epiphany self etch (SE) to the radicular dentin after removing the calcium hydroxide by hand file or ultrasonic methods. Materials and Methods: Thirty-six single-rooted human extracted teeth were used in this study. After root canal preparation, the teeth were divided into three groups. In group 1, as the control, no pretreatment was performed; while in groups 2 and 3, this was carried out using calcium hydroxide paste. After one week, the paste was re-moved with hand stainless steel K-files in group 2 and ultrasonic instrumentation in group 3. All samples were obturated with Resilon/Epiphany SE. One-millimeter slic-es of mid-root dentin were prepared for the push-out test (14 slices per group). After the bond strength was assessed, the failure modes were examined. The data were ana