RESEARCH ARTICLE Expression level of a flavo u

tio dins are not commonly found in higher plants [4], but sorghum accumulates them in response to pathogen infec-quired for 3-deoxyanthocyanidin synthesis have not been fully identified, and the key genes required for detemin-Mizuno et al. BMC Research Notes 2014, 7:761 http://www.biomedcentral.com/1756-0500/7/761or oligonucleotide array-based technology. Given theCenter, 1-2, Kannondai 2-chome, Tsukuba, Ibaraki 305-8602, Japan Full list of author information is available at the end of the articletion [1,5-7]. One 3-deoxyanthocyanidin, luteolinidin, is toxic to fungi and accumulates at increased levels in sorghum lines resistant to the anthracnose fungus [5,8]. Sorghum that accumulates 3-deoxyanthocyanidins ex-hibits various changes in coloration after infection with B. sorghicola [6]. The sorghum REDforGREEN mutant ing color variation remain to be elucidated. Functional genomic studies of sorghum began after its genome sequencing was completed in 2009 [10,11]. Whole-genome sequencing of sorghum BTx623 has re-vealed that many genes are duplicated and tandemly arrayed [10]. Each gene may have developed different functions related to a particular biochemical reaction. The sequence similarity of these duplicated genes makes it difficult to distinguish the expression of gene members of this family by using polymerase chain reaction (PCR)-* Correspondence