Research Article Oral Administration of Ganoderma lucidum to Lead-Exposed Rats Protects Erythrocytes against Hemolysis: Implicates to Anti-Anemia

Copyright © 2015 Shahdat Hossain et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We studied the effect of chronic oral exposure to lead acetate (PbA) on the sensitivity of RBC to hemolysis andwhether the sensitivity could be decreased by feeding the rats with extract ofmedicinalmushroomGanoderma lucidum.Three groups of rats, control, PbA-exposed, and G. lucidum (Gl)+PbA,were used. PbA (3mM) was administered via drinking water and G. lucidum extract by gavage at 300mg/Kg BW/day for 12 weeks. Afterwards, the rats were killed and washed RBCs were subjected to hemolysis in the presence of Fenton’s reagents. Hemolysis was determined by estimating the amount of released hemoglobin. The levels of lipid peroxide (LPO) and GSH were determined from RBC membranes and whole RBCs, respectively. The levels of TN