Physics, Faculty of Medi- cal Sciences, Tarbiat

modares.ac.ir Background: Various MTT assay methods are proposed to obtain the cell sur-vival parameters. Objective: Determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel MTT assay method after exposing them to ionizing radiation. Method: A common and a novel MTT assay method were used and compared for obtaining the F10B16 melanoma and 4T1 breast adenocarcinoma survivals after exposing them to ionizing radiation from a Co-60 machine. To obtain the survival parameters of the cells based on the common method, the cells were inoculated in 96-well plates. After irradiating the plates, the MTT assay was performed over the following days for a period of 8 days. Thereafter, the survival fraction was calcu-lated from a simple equation for every day from which the best day was selected. To acquire the cells ’ survival parameters based on the novel method, extensive experi