Mohammed et al. Microbial Cell Factories 2014, 13:102

Full list of author information is available at the end of the articlemolecule widely recognized in the field of biological sci-ences [1]. It is a water-soluble vitamin that functions as a cofactor essential in fat biochemical reactions, protein metabolism and hemoglobin synthesis as well as DNA synthesis and regulation. It comprises of two main as cyanocobalamin is a product formed by substituting adenosyl and methyl with a cyano group during the ex-traction process to ensure stability [4]. Industrial micro-bial fermentation is used as an alternative method to the originally established vitamin B12 chemical synthesis which requires at least 60 steps [5]. The natural process of vitamin B12 is synthesis by approximately 30 enzyme-mediated steps proceeding either through aerobically evident in Pseudomonas denitrificans, or anaerobically as wit