moshkovskii

Background: Entamoeba moshkovskii and E. dispar are impossible to differenti-ate microscopically from the pathogenic species E. histolytica. Multiplex poly-merase chain reaction (Multiplex PCR) is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment. Methods: For detection and differentiation of the three-microscopy indistin-guishable Entamoeba species in human, multiplex PCR assay using different DNA extraction methods was studied. A conserved forward primer was de-rived from the middle of the small-subunit rRNA gene, and reverse primers were designed from signature sequences specific to each of these three Enta-moeba species. Results: A 166-bp PCR product with E. histolytica DNA, a 580-bp product with E. moshkovskii DNA and a 752-bp product with E. dispar DNA were gen-erated in a single-round and multiplex PCR reaction. Conclusion: We recommend this PCR assay as an accurate, rapid, and effec