Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS

MS/MS method for the simultaneous determination of 25 key sphingolipid components in human plasma, in-cluding phosphorylated sphinganine and sphingosine, in a single 9-min run. This method enables an effective and high-throughput coverage of the metabolic changes involving the sphingolipidome during physiological or pathological states. The method is based on liquid–liq-uid extraction followed by reversed-phase LC-MS/MS. Exogenous odd-chain lipids are used as cost-effective but reliable internal standards. The method was fully validated in surrogate matrix and naive human plasma following FDA guidelines. Sample stability and dilution integrity were also tested and verified