High Sensitivity Detection of Active Botulinum Neurotoxin by Glyco- Quantitative Polymerase Chain-Reaction

  • Seok Joon Kwon
  • Eun Ji Jeong
  • Yung Choon Yoo
  • Chao Cai
  • Gi-hyeok Yang
  • Jae Chul Lee
  • Jonathan S. Dordick
  • Robert J. Linhardt
  • Kyung Bok Lee
Publication date
August 2016

Abstract

ABSTRACT: The sensitive detection of highly toxic botulinum neurotoxin (BoNT) from Clostridium botulinum is of critical importance because it causes human illnesses if foodborne or introduced in wounds and as an iatrogenic substance. Moreover, it has been recently considered a possible biological warfare agent. Over the past decade, significant progress has been made in BoNT detection technologies, including mouse lethality assays, enzyme-linked immunosorbent assays, and endopeptidase assays and by mass spectrometry. Critical assay requirements, including rapid assay, active toxin detection, sensitive and accurate detection, still remain challenging. Here, we present a novel method to detect active BoNTs using a Glyco-quantitative polymeras...

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