Original Article A Comparative Study of Osteogenic Differentiation Human Induced Pluripotent Stem Cells and Adipose Tissue Derived Mesenchymal Stem Cells

Objective: Human induced pluripotent stem cells (iPSCs) have been shown to have promising capacity for stem cell therapy and tissue engineering applications. Therefore, it is essential to compare the ability of these cells with the commonly used mesenchymal stem cells (MSC) for bone tissue engineering in vitro. Materials and Methods: In this experimental study, the biological behavior and osteo-genic capacity of the iPSCs were compared with MSCs isolated from human adipose tis-sue (AT-MSCs) using 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Alizarin red staining, alkaline phosphatase (ALP) activity measurements, calcium content assay and common osteogenic-related genes. Data were reported as the mean ± SD. One-way analysis of variance (ANOVA) was used to compare the results. A p value of less than 0.05 was considered statistically significant. Results: There was a significant difference between the rate of proliferation of the two types of stem cells; iPSCs showed increased proliferation compared to AT-MSCs. Dur-ing osteogenic differentiation, ALP activity and mineralization were demonstrated to b