A. Brenkman: T. Hankemeier: J. van Duynhoven:

# The Author(s) 2012. This article is published with open access at Springerlink.com Abstract Oxylipins, including eicosanoids, affect a broad range of biological processes, such as the initiation and reso-lution of inflammation. These compounds, also referred to as lipid mediators, are (non-) enzymatically generated by oxida-tion of polyunsaturated fatty acids such as arachidonic acid (AA). A plethora of lipid mediators exist which makes the development of generic analytical methods challenging. Here we developed a robust and sensitive targeted analysis platform for oxylipins and applied it in a biological setting, using high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) operated in dynamic multiple reaction monitoring (dMRM). Besides the well-described AA metabolites, oxylipins derived from linoleic acid, dihomo-γ-linolenic acid, α-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid were included. Our comprehensive platform allows the quantitative evaluation of approximatel