Method Creation of targeted genomic deletions usi s

ente erm a b s t r a c t The use of TALEN and CRISPR/CAS nucleases is becoming increasingly popular as a means to edit sin-Thus, it is possible to produce mutants from one cell embryos, into TAL effector nucleases (TALEN) [4]. The latest, third generation erial defense sys-g) RNAs fo ards new two domain nuclease Cas9 and will guide the binary complex to the complementary DNA sequence for DSB induction. These DSBs are then either repaired by homologous recombination (HR) together with gene targeting vectors [6,7] or become, in the absence of repair templates, religated by non-homologous end joining (NHEJ). This is frequently accompanied by the loss of multi-ple nucleotides [8]. HR-mediated repair of nuclease-induced DSBs enables the insertion of preplanned sequence alterations into the nuclease