Research Article Proteomic Analysis of Human Brain Microvascular Endothelial Cells Reveals Differential Protein Expression in Response to Enterovirus 71 Infection

which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2DDIGE technologywas employed on proteins prepared fromhuman brainmicrovascular endothelial cells (HBMEC), to study the differentially expressed proteins in cells at 0 h, 1 h, 16 h, and 24 h after infection. Proteins found to be differentially expressed were identifiedwithmatrix-assisted laser desorption/ionization time-of-flight/time-of-flightmass spectrometry (MALDITOF/TOFMS) analysis. We identified 43 spots showing changes of at least 2.5 fold up- or downregulated expressions in EV71-infected cells at different timewhen comparing to control, and 28 proteins could be successfully identified byMALDI TOF/TOFmass spectrometry analysis. 4 proteins were significantly upregulated, and 6 proteins were downregulated, another 18 proteins were different expression at different incubation time.We identified changes in the expression of 12 cellularmetabolism-related proteins, 5molecules involved in cytoskeleton, 3 molecules involved in energy metabolism, 2 molecules involved in signal transduction, 1 molecule involved in the ubiquitin-proteasome pathway, 1 molecule involved in cell cycle, 1 molecule involved in apoptosis-related protein, 1 molecula